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transwell boyden chambers  (Corning Life Sciences)

 
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    Corning Life Sciences transwell boyden chambers
    Transwell Boyden Chambers, supplied by Corning Life Sciences, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/transwell+boyden+chambers/transwell+boyden+chamber/pmc12208135-182-7-11
    Average 90 stars, based on 1 article reviews
    transwell boyden chambers - by Bioz Stars, 2026-09
    90/100 stars

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    Related Articles

    Cell Migration Assay:

    Article Title: Silencing of transient receptor potential canonical channel 4 inhibits endothelial progenitor cell angiogenesis by suppressing VEGF and SDF-1
    Article Snippet: .. Cell migration assay To assess the migratory function of cells, Transwell Boyden chambers (8.0 μm, Corning, NY, USA) were utilized to quantify the effects of TRPC4 silencing on EPC migration. ..

    Migration:

    Article Title: Silencing of transient receptor potential canonical channel 4 inhibits endothelial progenitor cell angiogenesis by suppressing VEGF and SDF-1
    Article Snippet: .. Cell migration assay To assess the migratory function of cells, Transwell Boyden chambers (8.0 μm, Corning, NY, USA) were utilized to quantify the effects of TRPC4 silencing on EPC migration. ..

    Article Title: A thermosensitive hydrogel for the sustained delivery of exosomes extracted from menstrual blood mesenchymal stem cells and frizzled antibody on triple-negative breast cancer cells in vitro
    Article Snippet: Due to the heterogeneity of triple-negative breast cancer (TNBC), conventional treatments usually respond poorly and effective targeted therapies for this type of cancer are rare.. Wnt signaling plays an important role in breast cancer metastasis and proliferation, as shown by analyzes of gene expression profiling and genome-wide sequencing.. In addition, the role of endometrial cells in regulating angiogenesis during the menstrual cycle as an angiostatic state associated with the end of the cycle has been well established.

    Article Title: Silencing of transient receptor potential canonical channel 4 inhibits endothelial progenitor cell angiogenesis by suppressing VEGF and SDF-1
    Article Snippet: .. To assess the migratory function of cells, Transwell Boyden chambers (8.0 μm, Corning, NY, USA) were utilized to quantify the effects of TRPC4 silencing on EPC migration. ..

    Article Title: Qimai Feiluoping Decoction Inhibits EndMT to Alleviate Pulmonary Fibrosis by Reducing PI3K/AKT/mTOR Pathway-Mediated the Restoration of Autophagy
    Article Snippet: .. The migration of HUVECs was assessed using Transwell Boyden chambers from Corning Costar, Cambridge, USA, equipped with a polycarbonate filter with 8 μm pores. ..

    Transfection:

    Article Title: Hypoxia-induced PRMT1 methylates HIF2β to promote breast tumorigenesis via enhancing glycolytic gene transcription.
    Article Snippet: For trans-well assay, cells transfected with shRNAs were re-seeded on the top compartment of transwell Boyden chambers (8 mm, Corning, USA) in serum-free medium, and then were allowed to migrate to the lower compartment contained complete medium with 10% FBS (fetal bovine serum) in a humidified, 5% CO2 atmosphere incubator at 37 C. After 24 h, cells that did not migrate into the lower compartment were wiped away with a cotton swab. .. For trans-well assay, cells transfected with shRNAs were re-seeded on the top compartment of transwell Boyden chambers (8 mm, Corning, USA) in serum-free medium, and then were allowed to migrate to the lower compartment contained complete medium with 10% FBS (fetal bovine serum) in a humidified, 5% CO2 atmosphere incubator at 37 C. After 24 h, cells that did not migrate into the lower compartment were wiped away with a cotton swab. .. Extracellular lactic acid detection Extracellular lactic acid was detected by Lactic Acid assay kit (Nanjing Jiancheng Bioengineering Institute, A019-2-1) following the manufacturer’s protocol.

    Transwell Assay:

    Article Title: Estrogen-Induced LncRNA, LINC02568, Promotes Estrogen Receptor-Positive Breast Cancer Development and Drug Resistance Through Both In Trans and In Cis Mechanisms.
    Article Snippet: .. For Transwell assay, siRNA-transfected or lenti-viral-infected cells were re-seeded on the top compartment of Transwell Boyden chambers (8 μm, Corning, USA) in serum-free media, and then allowed to migrate to the lower compartment contained complete media with 10% FBS in a humidified, 5% CO2 atmosphere incubator at 37 °C. ..



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    a F4/80 + Mϕ among CD11b + cells (Supplementary Fig. ) in control and CXCR4 Mϕ-cKO mammary glands ( n = 6, 7, biologically independent samples). b F4/80 and K8 IF staining of control and CXCR4 Mϕ-cKO mammary glands. Enlarged views of boxed areas are shown. c Quantification of intraepithelial F4/80 + cells per field ( n = 4, biologically independent samples, 3 ductal areas were randomly selected per each section). d <t>Transwell</t> migration assays of control and CXCR4 Mϕ-cKO macrophages ( n = 4, biologically independent samples, 5 microscopic fields were randomly selected from each well). e Heatmap of differentially expressed genes (DEGs) related to macrophage migration from RNA-seq of control and CXCR4 Mϕ-cKO Mϕ. f Transwell migration of CXCR4 + and CXCR4 − macrophages ( n = 3, biologically independent samples, 5 microscopic fields were randomly selected). g Pathway enrichment analysis from DEGs in CXCR4 + vs. CXCR4 − macrophages, highlighting migration/Chemotaxis pathways ranked by adjusted FDR. h Quantification of Lin - CD24 + CD29 Hi Basal/MaSCs (P4) cells (Supplementary Fig. ) from control and CXCR4 Mϕ-cKO mammary glands ( n = 7, biologically independent samples). i Mammosphere formation of 5000 P4 cells from control and CXCR4 Mϕ-cKO mice ( n = 3, biologically independent samples). j Limited dilution assay with P4 cells from control and CXCR4 Mϕ-cKO mammary glands. Table representing serial dilution injections with the corresponding take rate and repopulation frequencies (calculated by ELDA, Pearson’s Chi-squared test, two-sided). k Representative Carmine alum-stained mammary outgrowths. l , m Mammosphere formation assay using 5000 P4 cells cocultured with l 20,000 Mϕ from control and CXCR4 Mϕ-cKO , or m 20,000 CXCR4 + and CXCR4 − Mϕ ( n = 3, biologically independent). n Limited dilution assay of WT P4 injected into control and CXCR4 Mϕ-cKO recipient mice; ductal branching quantified ( n = 7, biologically independent samples). o Representative mammary outgrowths. Scale bar, 50 μm in ( b ) and 25 μm in ( k ) and ( o ). Data are mean values ± s.d. Statistical significance was calculated by two-tailed unpaired Student’s t -test or one-way ANOVA with Turkey’s test. Box plots show the median (center line), 25th/75th percentiles (box bounds), whiskers extending to 1.5× IQR, and outliers plotted individually. Source data are provided as a Source Data file.
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    a F4/80 + Mϕ among CD11b + cells (Supplementary Fig. ) in control and CXCR4 Mϕ-cKO mammary glands ( n = 6, 7, biologically independent samples). b F4/80 and K8 IF staining of control and CXCR4 Mϕ-cKO mammary glands. Enlarged views of boxed areas are shown. c Quantification of intraepithelial F4/80 + cells per field ( n = 4, biologically independent samples, 3 ductal areas were randomly selected per each section). d <t>Transwell</t> migration assays of control and CXCR4 Mϕ-cKO macrophages ( n = 4, biologically independent samples, 5 microscopic fields were randomly selected from each well). e Heatmap of differentially expressed genes (DEGs) related to macrophage migration from RNA-seq of control and CXCR4 Mϕ-cKO Mϕ. f Transwell migration of CXCR4 + and CXCR4 − macrophages ( n = 3, biologically independent samples, 5 microscopic fields were randomly selected). g Pathway enrichment analysis from DEGs in CXCR4 + vs. CXCR4 − macrophages, highlighting migration/Chemotaxis pathways ranked by adjusted FDR. h Quantification of Lin - CD24 + CD29 Hi Basal/MaSCs (P4) cells (Supplementary Fig. ) from control and CXCR4 Mϕ-cKO mammary glands ( n = 7, biologically independent samples). i Mammosphere formation of 5000 P4 cells from control and CXCR4 Mϕ-cKO mice ( n = 3, biologically independent samples). j Limited dilution assay with P4 cells from control and CXCR4 Mϕ-cKO mammary glands. Table representing serial dilution injections with the corresponding take rate and repopulation frequencies (calculated by ELDA, Pearson’s Chi-squared test, two-sided). k Representative Carmine alum-stained mammary outgrowths. l , m Mammosphere formation assay using 5000 P4 cells cocultured with l 20,000 Mϕ from control and CXCR4 Mϕ-cKO , or m 20,000 CXCR4 + and CXCR4 − Mϕ ( n = 3, biologically independent). n Limited dilution assay of WT P4 injected into control and CXCR4 Mϕ-cKO recipient mice; ductal branching quantified ( n = 7, biologically independent samples). o Representative mammary outgrowths. Scale bar, 50 μm in ( b ) and 25 μm in ( k ) and ( o ). Data are mean values ± s.d. Statistical significance was calculated by two-tailed unpaired Student’s t -test or one-way ANOVA with Turkey’s test. Box plots show the median (center line), 25th/75th percentiles (box bounds), whiskers extending to 1.5× IQR, and outliers plotted individually. Source data are provided as a Source Data file.
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    Image Search Results


    a F4/80 + Mϕ among CD11b + cells (Supplementary Fig. ) in control and CXCR4 Mϕ-cKO mammary glands ( n = 6, 7, biologically independent samples). b F4/80 and K8 IF staining of control and CXCR4 Mϕ-cKO mammary glands. Enlarged views of boxed areas are shown. c Quantification of intraepithelial F4/80 + cells per field ( n = 4, biologically independent samples, 3 ductal areas were randomly selected per each section). d Transwell migration assays of control and CXCR4 Mϕ-cKO macrophages ( n = 4, biologically independent samples, 5 microscopic fields were randomly selected from each well). e Heatmap of differentially expressed genes (DEGs) related to macrophage migration from RNA-seq of control and CXCR4 Mϕ-cKO Mϕ. f Transwell migration of CXCR4 + and CXCR4 − macrophages ( n = 3, biologically independent samples, 5 microscopic fields were randomly selected). g Pathway enrichment analysis from DEGs in CXCR4 + vs. CXCR4 − macrophages, highlighting migration/Chemotaxis pathways ranked by adjusted FDR. h Quantification of Lin - CD24 + CD29 Hi Basal/MaSCs (P4) cells (Supplementary Fig. ) from control and CXCR4 Mϕ-cKO mammary glands ( n = 7, biologically independent samples). i Mammosphere formation of 5000 P4 cells from control and CXCR4 Mϕ-cKO mice ( n = 3, biologically independent samples). j Limited dilution assay with P4 cells from control and CXCR4 Mϕ-cKO mammary glands. Table representing serial dilution injections with the corresponding take rate and repopulation frequencies (calculated by ELDA, Pearson’s Chi-squared test, two-sided). k Representative Carmine alum-stained mammary outgrowths. l , m Mammosphere formation assay using 5000 P4 cells cocultured with l 20,000 Mϕ from control and CXCR4 Mϕ-cKO , or m 20,000 CXCR4 + and CXCR4 − Mϕ ( n = 3, biologically independent). n Limited dilution assay of WT P4 injected into control and CXCR4 Mϕ-cKO recipient mice; ductal branching quantified ( n = 7, biologically independent samples). o Representative mammary outgrowths. Scale bar, 50 μm in ( b ) and 25 μm in ( k ) and ( o ). Data are mean values ± s.d. Statistical significance was calculated by two-tailed unpaired Student’s t -test or one-way ANOVA with Turkey’s test. Box plots show the median (center line), 25th/75th percentiles (box bounds), whiskers extending to 1.5× IQR, and outliers plotted individually. Source data are provided as a Source Data file.

    Journal: Nature Communications

    Article Title: CXCR4 + mammary gland macrophageal niche promotes tumor initiating cell activity and immune suppression during tumorigenesis

    doi: 10.1038/s41467-025-59972-z

    Figure Lengend Snippet: a F4/80 + Mϕ among CD11b + cells (Supplementary Fig. ) in control and CXCR4 Mϕ-cKO mammary glands ( n = 6, 7, biologically independent samples). b F4/80 and K8 IF staining of control and CXCR4 Mϕ-cKO mammary glands. Enlarged views of boxed areas are shown. c Quantification of intraepithelial F4/80 + cells per field ( n = 4, biologically independent samples, 3 ductal areas were randomly selected per each section). d Transwell migration assays of control and CXCR4 Mϕ-cKO macrophages ( n = 4, biologically independent samples, 5 microscopic fields were randomly selected from each well). e Heatmap of differentially expressed genes (DEGs) related to macrophage migration from RNA-seq of control and CXCR4 Mϕ-cKO Mϕ. f Transwell migration of CXCR4 + and CXCR4 − macrophages ( n = 3, biologically independent samples, 5 microscopic fields were randomly selected). g Pathway enrichment analysis from DEGs in CXCR4 + vs. CXCR4 − macrophages, highlighting migration/Chemotaxis pathways ranked by adjusted FDR. h Quantification of Lin - CD24 + CD29 Hi Basal/MaSCs (P4) cells (Supplementary Fig. ) from control and CXCR4 Mϕ-cKO mammary glands ( n = 7, biologically independent samples). i Mammosphere formation of 5000 P4 cells from control and CXCR4 Mϕ-cKO mice ( n = 3, biologically independent samples). j Limited dilution assay with P4 cells from control and CXCR4 Mϕ-cKO mammary glands. Table representing serial dilution injections with the corresponding take rate and repopulation frequencies (calculated by ELDA, Pearson’s Chi-squared test, two-sided). k Representative Carmine alum-stained mammary outgrowths. l , m Mammosphere formation assay using 5000 P4 cells cocultured with l 20,000 Mϕ from control and CXCR4 Mϕ-cKO , or m 20,000 CXCR4 + and CXCR4 − Mϕ ( n = 3, biologically independent). n Limited dilution assay of WT P4 injected into control and CXCR4 Mϕ-cKO recipient mice; ductal branching quantified ( n = 7, biologically independent samples). o Representative mammary outgrowths. Scale bar, 50 μm in ( b ) and 25 μm in ( k ) and ( o ). Data are mean values ± s.d. Statistical significance was calculated by two-tailed unpaired Student’s t -test or one-way ANOVA with Turkey’s test. Box plots show the median (center line), 25th/75th percentiles (box bounds), whiskers extending to 1.5× IQR, and outliers plotted individually. Source data are provided as a Source Data file.

    Article Snippet: Cell migration was accessed using a Boyden-chamber transwell system with 8 μm pore polycarbonate membrane inserts (Corning).

    Techniques: Control, Staining, Migration, RNA Sequencing, Chemotaxis Assay, Dilution Assay, Serial Dilution, Tube Formation Assay, Injection, Two Tailed Test